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Etiology

Identification of Phytophthora Species by Disc Electrophoresis. H. S. Gill, Department of Plant Pathology, University of California, Riverside, CA 92521; G. A. Zentmyer, Department of Plant Pathology, University of California, Riverside, CA 92521. Phytopathology 68:163-167. Accepted for publication 20 July 1977. Copyright © 1978 The American Phytopathological Society, 3340 Pilot Knob Road, St. Paul, MN 55121. All rights reserved.. DOI: 10.1094/Phyto-68-163.

Soluble proteins from the mycelia of 30 isolates of Phytophthora cinnamomi, collected from 17 different hosts and from widely separated geographic locations, and of five isolates of P. cactorum, when fractionated by disc electrophoresis, yielded 22 and 26 bands with different densities. The two species differed markedly and each exhibited its distinct, characteristic protein pattern enabling us to identify them. With one exception, there was little or no variation in the protein patterns within the isolates of P. cinnamomi. Also, identical or nearly identical protein patterns of each species were obtained regardless of date of isolation, host, or geographic locality. No differences in protein patterns were seen between the mating types of P. cinnamomi.