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Completing the Life Cycle of Uromyces phaseoli var. typica on Bean Plants. J. V. Groth, Assistant professor, Department of Plant Pathology, University of Minnesota, St. Paul, MN 55108; B. D. Mogen, Laboratory technician, Department of Plant Pathology, University of Minnesota, St. Paul, MN 55108. Phytopathology 68:1674-1677. Accepted for publication 19 May 1978. Copyright © 1978 The American Phytopathological Society, 3340 Pilot Knob Road, St. Paul, MN 55121. All rights reserved.. DOI: 10.1094/Phyto-68-1674.

A technique was developed for germinating teliospores of the bean rust fungus, (Uromyces phaseoli var. typica), which allowed all five spore stages to develop on bean plants. Teliospores germinated on water-agar plates, beginning about 3-4 wk after a brief washing. Pycnia were obtained by inverting those plates over young bean plants in a greenhouse. Pycniospores were transferred between pycnia with sterile toothpicks, Aeciospores were collected, and host leaves were inoculated and incubated using the same methods as were used for urediospores. The technique has worked with many collections of the bean rust fungus that produced telia readily.