VIEW ARTICLE

Cytology and Histology

Ultrastructural Localization of Bean Common Mosaic Virus in Dormant and Germinating Seeds of Phaseolus vulgaris. H. C. Hoch, Department of Plant Pathology, New York State Agricultural Experiment Station, Cornell University, Geneva 14456; R. Provvidenti, Department of Plant Pathology, New York State Agricultural Experiment Station, Cornell University, Geneva 14456. Phytopathology 68:327-330. Accepted for publication 10 October 1977. Copyright © 1978 The American Phytopathological Society, 3340 Pilot Knob Road, St. Paul, MN 55121. All rights reserved.. DOI: 10.1094/Phyto-68-327.

The ultrastructural localization of bean common mosaic virus (BCMV) was determined for mature dehydrated and germinated Phaseolus vulgaris seeds. The BCMV particles appeared only as linear arrays in most cells of mature dehydrated seeds. Inclusions were present only as “scrolls”. Other virus-related inclusion material appeared as amorphous or fibrillar areas as well as paracrystalline arrays of undulated filaments. Following 48 hr of water imbibition and growth, meristem cells of the root apex were free of virus particles and virus-related inclusions. Pinwheel inclusions with plates were abundant beyond the 10th cell back from the root meristem initials. Individually dispersed BCMV particles became apparent in older cells of germinated seeds.