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Techniques

Technique for Axenic Production and Application of Cronartium fusiforme Basidiospores. H. V. Amerson, Research Associate, Department of Plant Pathology, North Carolina State University, Raleigh, NC 27607; R. L. Mott, Associate Professor of Botany, Department of Botany, North Carolina State University, Raleigh, NC 27607. Phytopathology 68:673-675. Accepted for publication 11 October 1977. Copyright © 1978 The American Phytopathological Society, 3340 Pilot Knob Road, St. Paul, MN 55121. All rights reserved.. DOI: 10.1094/Phyto-68-673.

A technique, which utilized excised nondisinfested telial columns of Cronartium fusiforme, was developed to deliver contaminant-free basidiospores to areas approximately 1.4 mm in diameter. The spores germinated and occasionally produced mycelia on modified Gresshoff and Doy medium 1. Pinus taeda seedlings also were infected with basidiospores in vitro.