VIEW ARTICLE

Disease Detection and Losses

Use of Sodium Dodecyl Sulfate in Serodiagnosis of Barley Stripe Mosaic Virus in Embryos and Leaves. T. W. Carroll, Professor, Department of Plant Pathology, Montana State University, Bozeman 59717; P. L. Gossel(2), and D. L. Batchelor(3). (2)(3)Former laboratory technician, and former assistant professor, Department of Plant Pathology, Montana State University, Bozeman 59717. Phytopathology 69:12-14. Accepted for publication 19 July 1978. Copyright 1979 The American Phytopathological Society. DOI: 10.1094/Phyto-69-12.

Sodium dodecyl sulfate (SDS) was used in the serodiagnosis of barley stripe mosaic virus (BSMV) in embryos and leaves. Antisera elicited by SDS-treated preparations of partially purified BSMV were produced in rabbits and then used in an agar gel immunodiffusion medium amended with SDS. The serodiagnostic test was based on a modified Ouchterlony double-diffusion technique in which SDS replaced Leonil SA as the viral degrading agent. Filter paper disks served as seroreactant depots. The SDS-disk test worked equally well with freshly prepared or lyophilized seroreactants. More than 50,000 embryos were assayed successfully. The procedure shows promise for field surveys and seed testing worldwide.