VIEW ARTICLE

Techniques

Reuse of Coated Enzyme-Linked Immunosorbent Assay Plates. M. Bar -Joseph, Virus Laboratory, Agricultural Research Organization, Bet Dagan, Israel; Mira Moscovitz(2), and Yehudit Sharafi(3). (2)(3)Virus Laboratory, Agricultural Research Organization, Bet Dagan, Israel. Phytopathology 69:424-426. Accepted for publication 1 September 1978. Copyright 1979 The American Phytopathological Society. DOI: 10.1094/Phyto-69-424.

The dissociation reactions of four plant viruses: citrus tristeza virus (CTV), carnation mottle virus (CarMV), carnation yellow fleck virus (CYFV), and tobacco mosaic virus (TMV) and their respective γ-globulin alkaline phosphatase conjugates, sandwiched to antibody microplates, were examined. Treatment with 0.2 M glycine-HCl buffer pH 2.2 for 60 min caused the double antibody sandwiches of CTV and CarMV to dissociate from the antibody-coated microplates. In similar treatments CYFV was eluted less efficiently. The TMV double-sandwich remained undissociated by acidification but it could be partially dissociated under alkaline conditions (pH 12.1). The application of microplate recycling for economizing routine large-scale screening of CTV is described.