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Etiology

Isolation and Characterization of Peanut Stunt Virus from Alfalfa in Spain. J. R. Diaz-Ruiz, Plant Virology Laboratory, Plant Protection Institute, Agricultural Research, Science and Education Administration, U.S. Department of Agriculture, Beltsville, MD 20705, Present address: Departamento de Virología, Instituto “Jaime Ferrán,” Joaquin Costa 32, Madrid 6, Span; J. M. Kaper(2), H. E. Waterworth(3), and J. C. Devergne(4). (2)Plant Virology Laboratory, Plant Protection Institute, Agricultural Research, Science and Education Administration, U.S. Department of Agriculture, Beltsville, MD 20705; (3)Plant Introduction Station, USDA-SEA/AR, Glenn Dale, MD 20769; (4)Station de Botanique et Pathologie Vegetale, Centre de Recherches d’Antibes, France. Phytopathology 69:504-509. Accepted for publication 23 November 1978. This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 1979. DOI: 10.1094/Phyto-69-504.

A cucumovirus was isolated from alfalfa (Medicago sativa) growing in the Barajas area near Madrid, Spain. After purification, the virus was identified as a strain of peanut stunt virus (PSV). In its host range, the virus (designated PSV-B) resembled previously characterized PSV strains (T, V, and W) from the United States with only minor symptom differences in a few plant species. Serologically, PSV-B was indistinguishable from PSV-W and was closely related, but not identical, to PSV-V. There was a more distant serologic relationship between PSV-B and some strains of cucumber mosaic virus (CMV) and tomato aspermy virus. Although the sedimentation rate of PSV-B was identical to previously characterized cucumoviruses, in polyacrylamide gel electrophoresis PSV-B migrated with a different mobility than either PSV-V or PSV-W, although the difference from PSV-W was very small. It coelectrophoresed with CMV strain D. The virus is different from most other cucumoviruses in that it is very unstable in solution, where it degraded into nucleoprotein particles with bacilliform structures and lower sedimentation rates. These nucleoproteins contained the separate viral RNA components. Base ratios of the RNAs of PSV-B, PSV-W, and PSV-V revealed great similarity but were significantly different from that of CMV.