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Etiology

Properties of Soybean Mosaic Virus Ribonucleic Acid. J. H. Hill, Department of Plant Pathology, Seed, and Weed Science, Iowa State University, Ames, 50011; H. I. Benner, Department of Plant Pathology, Seed, and Weed Science, Iowa State University, Ames, 50011. Phytopathology 70:236-239. Accepted for publication 24 August 1979. Copyright 1980 The American Phytopathological Society. DOI: 10.1094/Phyto-70-236.

Infectious single-stranded RNA isolated from purified soybean mosaic virus has an S value of 39.7 before, and 25.4 after, formaldehyde denaturation. Nucleic acid molecular-weight determinations made by linear log sucrose density gradient centrifugation gave values of 3.46 × 10⁶ and 3.18 × 10⁶, respectively, for native and formaldehyde-treated RNA. Electrophoresis on polyacrylamide gels gave molecular weight values of 3.02 × 10⁶ both before and after formaldehyde denaturation. No evidence for virion-associated low molecular weight RNAs was obtained. Thermal denaturation profiles showed at T_m of 49 C in 0.1 M sodium phosphate, pH 7.0, and a hyperchromicity of 30.4%. Nucleotide composition was 29.9% adenylic acid, 24.3% guanylic acid, 14.9% cytidylic acid, and 30.9% uridylic acid. The RNA is 5.3% of the virus particle.