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Techniques

A Method for Long-time Culture Storage of Rhizoctonia solani. Edward E. Butler, Professor, Department of Plant Pathology, University of California, Davis 95616; Phytopathology 70:820-821. Accepted for publication 22 February 1980. Copyright 1980 The American Phytopathological Society. DOI: 10.1094/Phyto-70-820.

A method is described for long-time preservation of cultures of Rhizoctonia solani in tubes in a dry soil containing 4% (w/w) wheat bran. The moisture level of the mixture at the time of inoculation with R. solani was 0.23 g water per gram (dry weight) of soil. About 1 mo at 23–27 C was required to allow the colonized mix to air dry; then it was stored at –25 C. Isolates from diverse sources survived 55 mo at –25 C. Virulence, morphology, and cultural characteristics of R. solani were not changed.