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Physiology and Biochemistry

Virus Infection of Trifolium Species in Cell Suspension Cultures. R. A. Jones, Department of Agronomy, Clemson University, Clemson, SC 29631; E. A. Rupert(2), and O. W. Barnett(3). (2)Department of Agronomy and Soils, Clemson University, Clemson, SC 29631; (3)Department of Plant Pathology and Physiology, Clemson University, Clemson, SC 29631. Phytopathology 71:116-119. Accepted for publication 25 June 1980. Copyright 1981 The American Phytopathological Society. DOI: 10.1094/Phyto-71-116.

Cell suspensions derived from callus tissue cultures of Trifolium repens L., T. ambiguum Bieb., T. hybridum L., and the hybrid T. ambiguum × T. hybridum were inoculated with clover yellow mosaic virus (CYMV) or clover yellow vein virus (CYVV) and analyzed for ability to support virus multiplication. Inoculations with purified viruses were made 24 hr after cell subculture. Samples of inoculated cells, taken 6 and 12 hr later and thereafter at 24-hr intervals through 144 hr, were examined for the presence of inclusion bodies, and virus concentration was determined by latex serology. CYMV multiplication occurred in cultures of all species, but concentrations were lower in T. ambiguum and the hybrid. When cultures were inoculated with CYVV, T. ambiguum and the hybrid did not support virus multiplication, while T. repens and T. hybridum produced serologically detectable virus. These in vitro responses to both viruses closely approximated responses to in situ plant inoculations.