VIEW ARTICLE

Physiology and Biochemistry

Simultaneous Detoxification of Phytoalexins by Fusarium solani f. sp. phaseoli. D. A. Smith, Plant Pathology Department, University of Kentucky, Lexington 40546; J. M. Harrer, and T. E. Cleveland, Plant Pathology Department, University of Kentucky, Lexington 40546. Phytopathology 71:1212-1215. Accepted for publication 13 March 1981. Copyright 1981 The American Phytopathological Society. DOI: 10.1094/Phyto-71-1212.

The isoflavonoid phytoalexins, kievitone and phaseollidin, added to liquid cultures of Fusarium solani f. sp. phaseoli, were simultaneously detoxified to kievitone hydrate and phaseollidin hydrate, respectively. Both phytoalexins (each at 20 μg/ml) were transformed within 24 hr after being added to fungal cultures. No increase in mycelial dry weight of F. solani f. sp. phaseoli occurred while detoxification was proceeding. A purified preparation of kievitone hydratase, the enzyme responsible for kievitone transformation, did not accomplish simultaneous detoxification; kievitone was detoxified, but phaseollidin was not.