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A Rapid Staining Method for Erysiphe graminis f. sp. hordei in and on Whole Barley Leaves with a Protein-Specific Dye. Gerhard Wolf, Institut für Pflanzenpathologie und Pflanzenschutz, University of Göttingen, Grisebachstrasse 6, D-3400 Göttingen, West Germany; Friedrich Fric, Institut für Pflanzenpathologie und Pflanzenschutz, University of Göttingen, Grisebachstrasse 6, D-3400 Göttingen, West Germany, Present address: Institute of Experimental Biology and Ecology, Bratislava, CSSR. Phytopathology 71:596-598. Accepted for publication 4 November 1980. Copyright 1981 The American Phytopathological Society. DOI: 10.1094/Phyto-71-596.

Whole barley leaves infected with Erysiphe graminis f. sp. hordei were fixed with and cleared in ethanol/chloroform/trichloroacetic acid and stained with Coomassie Brilliant Blue R-250. Conidia, germ tubes, and haustoria as well as reactions of the host cell could be visualized by this protein-specific dye.