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Vector Relations

Inoculation of the Aphids Hyperomyzus lactucae and Chaetosiphon jacobi with Isolates of Sowthistle Yellow Vein Virus and Strawberry Crinkle Virus. Edward S. Sylvester, Professor of entomology, Department of Entomological Sciences, University of California, Berkeley 94720; Jean Richardson, staff research associate, Department of Entomological Sciences, University of California, Berkeley 94720. Phytopathology 71:598-602. Accepted for publication 10 November 1980. Copyright 1981 The American Phytopathological Society. DOI: 10.1094/Phyto-71-598.

Injection of the propagative plant rhabdoviruses, sowthistle yellow vein virus (SYVV) and strawberry crinkle virus (SCV) into the aphids Hyperomyzus lactucae and Chaetosiphon jacobi demonstrated that the viruses could replicate in either aphid species when introduced alone or in sequence. There was no evidence of cross-protection or interference. SCV, when infecting H. lactucae, was not transmitted to sowthistle, Sonchus oleraceus, nor was SYVV transmitted to strawberry, Fragaria vesca, by C. jacobi. Host specificity of the aphids prevented complete vector transmission tests. Three serial passages of SYVV in C. jacobi did not alter its infectivity for H. lactucae or its transmissibility to sowthistle by that species. There was some evidence that C. jacobi was less efficient in propagating SYVV than was the normal host vector, H. lactuace. Electron microscopic examination of thin sections of the two aphid species demonstrated that enveloped and nonenveloped particles of SCV could be found in the cytoplasm of the organs and tissues of both aphid species. SYVV was found in nuclei and cytoplasm of all major organ systems of the host vector, H. lactucae, but in C. jacobi virions were found only in the nuclei of fat body or tracheole cells or in cells near the cuticle in the tonofilament-muscle attachment area.