VIEW ARTICLE

Genetics

Use of P-1 Incompatibility Group Plasmids to Introduce Transposons into Pseudomonas solanacearum. Christian Boucher, Pathologie végétale I.N.R.A., Route de Saint-Cyr, 78000 Versailles, France, Present address: Laboratoire de Biologie Moléculaire des Relations Plantes—Microorganismes. INRA Chemin de Borde-Rouge-Auzeville. 31320 Castanet Tolosan BP 12; Brigitte Message(2), Danièle Debieu(3), and Claudine Zischek(4). (2)(3)(4)Pathologie végétale I.N.R.A., Route de Saint-Cyr, 78000 Versailles, France. Phytopathology 71:639-642. Accepted for publication 7 November 1980. Copyright 1981 The American Phytopathological Society. DOI: 10.1094/Phyto-71-639.

Strain K60 of Pseudomonas solanacearum cannot stably maintain P-1 incompatibility group plasmids. However, it is possible to isolate derivatives that receive and maintain these plasmids. This system has been used to mutagenize the strain with transposons Tn5, Tn7, and Tn10. In P. solanacearum. Tn5 and Tn10 are transposed with a relatively high frequency (5 × 10^–6), and 1% of the transposon-harboring clones obtained were auxotrophs. The transposition frequency with Tn7 is low.