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Physiology and Biochemistry

Victorin Production Stimulated by Oat Flakes. Harry Wheeler, Professor, Department of Plant Pathology, University of Kentucky, Lexington 40506; Donald Gantz, research associate, Department of Plant Pathology, University of Kentucky, Lexington 40506. Phytopathology 71:853-854. Accepted for publication 13 January 1981. Copyright 1981 The American Phytopathological Society. DOI: 10.1094/Phyto-71-853.

Fries’ solution, often supplemented with yeast extract, has served for many years as a standard medium for production of victorin by Helminthosporium victoriae and of selective pathotoxins by several other fungal pathogens. Adding oat flakes to Fries’ solution eliminated the need for yeast extract and greatly increased toxin titer. In media containing flakes, maximum toxin titers were achieved in 10–12 days instead of 3–4 wk, and residual dry weights of culture fluids were reduced by 30–50%.