VIEW ARTICLE

Techniques

Detection of Pathogenic Strains of Soybean Mosaic Virus by Enzyme-Linked Immunosorbent Assay with Polystyrene Plates and Beads as the Solid Phase. Li- Ching Chen, Graduate assistant, Department of Microbiology, Seed and Weed Sciences, Iowa State University, Ames 50011, Present address: Malaysian Agricultural Research and Development Institute (MARDI), Serdang, Selangor, Malaysia; D. P. Durand(2), and J. H. Hill(3). (2)Professor, Department of Microbiology, Seed and Weed Sciences, Iowa State University, Ames 50011; (3)Associate professor, Department of Plant Pathology, Seed and Weed Sciences, Iowa State University, Ames 50011. Phytopathology 72:1177-1181. Accepted for publication 8 February 1982. Copyright 1982 The American Phytopathological Society. DOI: 10.1094/Phyto-72-1177.

Optimal conditions were determined for an enzyme-linked immunosorbent assay system to detect soybean mosaic virus (SMV) by using polystyrene beads as the solid phase. The bead system was more discriminating for detection of serological differences among SMV isolates than a similar system using polystyrene microtitration plates as the solid phase. Seven SMV strains were classified into two, and possibly three, serological groups that correlated with virulence of the strains.