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Disease Detection and Losses

Ilar-Like Characteristics of American Plum Line Pattern Virus and Its Serological Detection in Prunus. Robert W. Fulton, Department of Plant Pathology, University of Wisconsin, Madison 53706; Phytopathology 72:1345-1348. Accepted for publication 29 March 1982. Copyright 1982 The American Phytopathological Society. DOI: 10.1094/Phyto-72-1345.

Purified American plum line pattern virus (AmPLV) retained infectivity in 0.02 M EDTA, pH 6.2, for 12–24 mo at 2 C. In other than ammonium phosphate, purified virus lost most of its infectivity at pH 7.0 or 8.0 in 2 hr at 24 C. AmPLV and a number of other viruses lost infectivity within a few minutes in potassium phosphate buffer at pH 8.0 if it had been prepared for more than 1 mo. In sucrose density gradients prepared with EDTA, AmPLV showed four components with sedimentation coefficients of about 95, 100, 114, and 126S. Particles of these components averaged 26, 28, 31, and 33 nm in diameter. All four components appeared to contribute to infectivity. In agar gel double diffusion serological tests, AmPLV was detectable in extracts of herbaceous, but not Prunus, hosts. Enzyme-linked immunosorbent assays gave positive tests on symptomless Prunus leaves. Buds from dormant material gave clear-cut test results.