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Growth Medium for Dual Cultures of Loblolly Pine Callus and Cronartium fusiforme. William R. Jacobi, Graduate research assistant, Department of Plant Pathology, North Carolina State University, Raleigh 27650, Current address: Department of Botany and Plant Pathology, Colorado State University, Fort Collins 80523; Phytopathology 72:136-138. Accepted for publication 26 March 1981. Copyright 1982 The American Phytopathological Society. DOI: 10.1094/Phyto-72-136.

A medium was developed for the simultaneous culture of loblolly pine callus and Cronartium fusiforme of basidiospore origin. Gresshoff and Doy medium 1 was modified by using different nitrogen concentrations, growth regulators, 2% glucose, adding 0.05% peptone, and 0.05% of an 80% ethanol-soluble fraction of yeast extract. This medium permitted good growth of both cultures without serious detriment to either organism during interactive studies. Light and temperature conditions suitable for survival and growth of both organisms are given.