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Cross-Reaction of Corynebacterium sepedonicum Antisera With C. insidiosum, C. michiganense, and an Unidentified Coryneform Bacterium. S. H. De Boer, Research scientist, Agriculture Canada Research Station, 6660 N.W. Marine Drive, Vancouver, British Columbia V6T 1X2; Phytopathology 72:1474-1478. Accepted for publication 14 April 1982. Copyright 1982 Department of Agriculture, Government of Canada.. DOI: 10.1094/Phyto-72-1474.

The immunoglobulin G (IgG) component of antiserum produced in rabbits against glutaraldehyde-fixed cells of Corynebacterium sepedonicum was purified by column chromatography on Sephacryl S300 and used for indirect immunofluorescence staining. Specific activity (immunofluorescence titer per milligram of protein) of IgG fractions was determined with C. sepedonicum, C. insidiosum, C. michiganense, and an unidentified coryneform bacterium isolated from a potato stem. Specific activity with C. sepedonicum was greater than with the heterologous bacteria in antisera obtained up to 20 wk after beginning immunization, but thereafter was about equal to or less than the reaction with the cross-reacting species. An antigenic fraction was extracted from acetone-dried C. sepedonicum cells with hot phenol and antigenic activity was detected in culture filtrates by agar double diffusion. The culture filtrate but not the phenol extract sensitized sheep erythrocytes in indirect hemagglutination.