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Genetics

Polypeptide Mapping by Two-Dimensional Electrophoresis and Pathogenic Variation in Field Isolates and Induced Mutants of Erysiphe graminis f. sp. tritici. D. W. Gabriel, Former graduate research assistant, Program in Genetics and Department of Botany and Plant Pathology, Michigan State University, East Lansing 48824, Present address: Department of Plant Pathology, Oklahoma State University, Stillwater 74074; A. H. Ellingboe, professor, Program in Genetics and Department of Botany and Plant Pathology, Michigan State University, East Lansing 48824, Present address: International Plant Research Institute, 853 Industrial Road, San Carlos, CA 94070. Phytopathology 72:1496-1499. Accepted for publication 3 May 1982. Copyright 1982 The American Phytopathological Society. DOI: 10.1094/Phyto-72-1496.

Methods were developed to extract proteins from the mycelium and conidia of the obligate parasite Erysiphe graminis f. sp. tritici and the facultative parasite Colletotrichum lindemuthianum. These methods resulted in preparations suitable for separation by two-dimensional electrophoresis. Over 600 polypeptides were routinely visualized when stained with silver. Proteins were extracted from C. lindemuthianum grown in shake culture. Polypeptide maps of a C. lindemuthianum mutant and the wild type showed differences in the positions of as many as 10% of the polypeptides. Proteins were extracted from E. graminis f. sp. tritici grown on wheat, without any detectable extraction of the wheat leaf proteins. Polypeptide maps of three E. graminis f. sp. tritici mutants with increased virulence were indistinguishable from each other and from wild-type culture MS-1. The two field isolates, MS-1 and MO-10, had unique polypeptide maps that differed by five polypeptides. Independent segregations of the polypeptide differences and three known P gene differences were observed in progeny of a cross between MS-1 and MO-10.