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Resistance

Association of Hypersensitive Host Cell Death and Autofluorescence with a Gene for Resistance to Peronospora manshurica in Soybean. T. Érsek, Institute for Plant Protection, Budapest, Hungary; M. Holliday(2), and N. T. Keen(3). (2)(3)Department of Plant Pathology, University of California, Riverside 92521, (2)Current address: Biochemistry Department, Experimental Station, E. I. du Pont de Nemours, Wilmington, DE 19898. Phytopathology 72:628-631. Accepted for publication 25 August 1981. Copyright 1982 The American Phytopathological Society. DOI: 10.1094/Phyto-72-628.

Leaves of a soybean cultivar carrying the Rpm allele for resistance to Peronospora manshurica, but not those of a near-isogenic susceptible cultivar, microscopically reacted to infection with a typical hypersensitive response, but the leaves did not exhibit visible symptoms. Necrotic host cell death occurred around infection sites in the resistant genotype based on epifluorescence observations of sodium fluorescein uptake. Autofluorescent material, possibly the phytoalexin glyceollin or related compounds, also accumulated in hypersensitive host cells surrounding fungal penetration sites. The results therefore indicated that resistance conferred by the Rpm allele is associated with a classical hypersensitive reaction.