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Techniques

Regeneration of Virus-Free Plants from Yellow-Green Areas and TMV-Induced Enations of Nicotiana tomentosa. James L. White, Department of Plant Pathology, University of Nebraska, Lincoln 68583-0722, Current address: National Research Council of Canada, Prairie Regional Laboratory, Saskatoon, Sask, S7N 0W9 Canada. Nebraska Agricultural Experiment Station Journal Paper 6658; Phytopathology 72:866-867. Accepted for publication 23 November 1981. Copyright 1982 The American Phytopathological Society. DOI: 10.1094/Phyto-72-866.

TMV-infected Nicotiana tomentosa plants formed enations at a low frequency on the undersides of several systemically infected leaves. About 60% of the plantlets regenerated from enations were virus-free. Unexpectedly, about one-third of the plants regenerated from yellow-green areas were virus-free.