VIEW ARTICLE

Techniques

Grouping of Seven Biologically Defined Isolates of Cucumber Mosaic Virus by Peptide Mapping. Michael C. Edwards, Former graduate research assistant, Department of Plant Pathology, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; Dennis Gonsalves, associate professor, Department of Plant Pathology, Cornell University, New York State Agricultural Experiment Station, Geneva 14456. Phytopathology 73:1117-1120. Accepted for publication 23 February 1983. Copyright 1983 The American Phytopathological Society. DOI: 10.1094/Phyto-73-1117.

Coat proteins of seven biologically defined isolates of cucumber mosaic virus were compared by peptide mapping and enzyme-linked immunosorbent assay (ELISA). All seven isolates could be classified in either of two subgroups, confirming previous reports of the existence of these groups. Isolates B, C-1, C-2, and F were placed in one subgroup, while isolates L-2, L-3, and WL were placed in the other. Isolates within a subgroup were indistinguishable. One-dimensional peptide mapping appears to be a reliable and sensitive technique that can complement existing viral classification techniques.