VIEW ARTICLE

Techniques

Predicting Potential Fungicide Resistance in Fungal Populations by Using a Continuous Culturing Technique. W. J. McPhee, Research scientist, Agriculture Canada, Research Station, Summerland, British Columbia V0H 1Z0; Earle R. Nestmann, Research scientist, Agriculture Canada, Research Station, Summerland, British Columbia V0H 1Z0. Phytopathology 73:1230-1233. Accepted for publication 1 April 1983. Copyright 1983 Department of Agriculture, Government of Canada. DOI: 10.1094/Phyto-73-1230.

A chemostat containing a continuously growing yeast population was used to study the development of resistance to pesticides after exposure to the fungicides, benomyl, CGA64251, or captan. The yeast population was dramatically reduced in response to each fungicide. Populations treated with benomyl recovered after approximately 2 days. The recovered population was resistant to benomyl and the individual resistant cells had a slower growth rate than the wild type, benomyl-sensitive cells. There was no recovery in populations treated with CGA64251 or captan.