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A Comparison of Three Potyviruses by Direct Hybridization Analysis. B. B. Reddick, Graduate research assistant, Department of Plant Pathology and Physiology, Clemson University, Clemson 29631; O. W. Barnett, professor, Department of Plant Pathology and Physiology, Clemson University, Clemson 29631. Phytopathology 73:1506-1510. Accepted for publication 11 April 1983. Copyright 1983 The American Phytopathological Society. DOI: 10.1094/Phyto-73-1506.

Viral RNAs extracted from several potyviruses (bean yellow mosaic [BYMV-Scott], pea mosaic [PMV-204-1 and PMV-Pratt], and clover yellow vein [CYVV-C-81 and CYVV-Pratt]) were compared by using direct molecular hybridization. Complementary DNA was synthesized from the RNA templates with reverse transcriptase, and hybridization of the various RNA:cDNA combinations was analyzed. The two CYVV isolates and two PMV isolates showed partial sequence homology to the BYMV-Scott isolate. Partial sequence homology between PMV-204-1 and PMV-Pratt or between CYVV-C-81 and CYVV-Pratt was demonstrated; however, no sequence homology was found when either of the PMV isolates was compared with either of the CYVV isolates. In the cytoplasmic inclusion body subdivision II, several viruses are distinguished by the formation of nuclear crystalline inclusions. BYMV, PMV, and CYVV should be included in this category as different, but related, viruses on the basis of these cDNA data and previous biological and biochemical data.