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Genetics

Genetic Analysis of Cucumber Mosaic Virus in Relation to Host Resistance: Location of Determinants for Pathogenicity to Certain Legumes and Lactuca saligna. Michael C. Edwards, Former graduate research assistant, Department of Plant Pathology, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; Dennis Gonsalves(2), and Rosario Provvidenti(3). (2)(3)Associate professor, and senior research associate, respectively, Department of Plant Pathology, Cornell University, New York State Agricultural Experiment Station, Geneva 14456. Phytopathology 73:269-273. Accepted for publication 5 August 1982. Copyright 1983 The American Phytopathological Society. DOI: 10.1094/Phyto-73-269.

Pseudorecombinants of two naturally occurring strains of cucumber mosaic virus (CMV-B and CMV-LsS) were used to analyze the genetics of CMV in relation to pathogenicity to Lactuca saligna, Phaseolus vulgaris, Pisum sativum, and Vigna unguiculata. RNA 2 of CMV-B determined systemic infection of bean, pea, and cowpea. Ability to systemically infect L. saligna depended upon both RNAs 2 and 3 of CMV-LsS. Chlorotic and necrotic local lesion formation on V. unguiculata depended upon RNA 2 from the B and LsS strains, respectively. A simple and effective procedure using bentonite for the recovery of infectious RNA from polyacrylamide gels is also described.