VIEW ARTICLE

Cytology and Histology

Cytoplasmic Cylindrical and Nucleolar Inclusions Induced by Potato Virus-A. J. R. Edwardson, Agronomist, University of Florida, Institute of Food and Agricultural Science, Agronomy Department, Potato Virus Laboratory, Gainesville 32611; R. G. Christie, plant pathologist III, University of Florida, Institute of Food and Agricultural Science, Agronomy Department, Potato Virus Laboratory, Gainesville 32611. Phytopathology 73:290-293. Accepted for publication 13 September 1982. Copyright 1983 The American Phytopathological Society. DOI: 10.1094/Phyto-73-290.

Cytoplasmic cylindrical and lacunose nucleolar inclusions induced by potato virus-A (PVA) in cells of Nicotiana clevelandii were studied by light and electron microscopy. Differential staining with Azure A and Calcomine-Orange, Luxol Brilliant Green revealed that components of these inclusions were proteinaceous. Cylindrical inclusions appeared in thin sections as pinwheels, scrolls, and short, curved laminated aggregates (subdivision-IV type inclusions). Neither cytoplasmic nor nucleolar inclusions were observed in healthy cells. The nucleolar inclusions induced by PVA are distinctive and can be used to separate infections caused by PVA from those caused by other potyviruses.