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Etiology

Comparison of Potato Yellow Dwarf Virus Serotypes. B. W. Falk, Assistant professor, University of Florida, Agricultural Research and Education Center, Belle Glade 33430; L. G. Weathers, professor, Department of Plant Pathology, University of California, Riverside 92521. Phytopathology 73:81-85. Accepted for publication 1 June 1982. Copyright 1983 The American Phytopathological Society. DOI: 10.1094/Phyto-73-81.

Three isolates of potato yellow dwarf virus (PYDV) were compared. The California (SYDV-CA) and New York (SYDV-NY) isolates of PYDV were representative of the SYDV serotype, while the New Jersey isolate (CYDV-NJ) represented the CYDV serotype. Serotype differences were readily observable by immunodiffusion and double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) tests, but were less apparent by indirect ELISA. However, SDS polyacrylamide gel electrophoresis of virion proteins showed distinct molecular weight differences for at least two virion proteins between the two PYDV serotypes. Proteins G and M₂ of CYDV-NJ had molecular weights of 92,500 and 29,000, respectively, compared to 85,000 and 24,000 for the corresponding proteins of the SYDV-CA and SYDV-NY serotypes. Despite their physical differences, the G proteins of the two serotypes cross reacted in indirect ELISA. Virion protein molecular weights, as well as serological properties, can be used to separate PYDV serotypes.