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Purification and Serology of Peanut Mottle Virus. Sue A. Tolin, Associate professor, Department of Plant Pathology and Physiology, Virginia Polytechnic Institute and State University, Blacksburg 24061; Rosemary H. Ford, former laboratory specialist, Department of Plant Pathology and Physiology, Virginia Polytechnic Institute and State University, Blacksburg 24061. Phytopathology 73:899-903. Accepted for publication 18 January 1983. Copyright 1983 The American Phytopathological Society. DOI: 10.1094/Phyto-73-899.

Peanut mottle virus (PMV) was purified from pea (Pisum sativum ‘Little Marvel’) by extraction in 0.1 M tris-HCl, pH 8.0, containing 0.05 M EDTA and 0.02 M Na₂SO₃, followed by clarification with 25% chloroform and precipitation with polyethylene glycol. Virus suspended in 0.01 M tris, pH 8.0, containing 0.5 M urea and 0.001 M EDTA sedimented primarily as a single component. Yields of 10- 25 mg/kg of tissue were obtained. Both yield and infectivity were enhanced by Na₂SO₃ and EDTA. Specific antisera, with microprecipitin titers of 1/256, reacted with PMV-infected pea, soybean, and peanut from the greenhouse or field in agar immunodiffusion tests containing sodium dodecyl sulfate (SDS). Purified PMV at 0.2 mg/ml was completely degraded at SDS concentrations greater than 0.05% in 0.005 M tris-HCl, pH 8.0. PMV was not related serologically to bean yellow mosaic, clover yellow vein, or soybean mosaic viruses as shown by SDS gels or ELISA.