VIEW ARTICLE

Techniques

Gel Electrophoresis of Virus-Associated Polypeptides for Detecting Viruses in Bulbous Irises. Miriam Alper, Virus Laboratory, Agricultural Research Organization, The Volcani Center, Bet Dagan 50-250, Israel; R. Salomon(2), and G. Loebenstein(3). (2)(3)Virus Laboratory, Agricultural Research Organization, The Volcani Center, Bet Dagan 50-250, Israel. Phytopathology 74:960-962. Accepted for publication 9 February 1984. Copyright 1984 The American Phytopathological Society. DOI: 10.1094/Phyto-74-960.

Gel electrophoresis of virus-associated polypeptides enabled the detection and differentiation of three potyviruses, including iris mild mosaic virus (IMMV), iris severe mosaic virus (ISMV), and bean yellow mosaic virus (BYMV), and a carlavirus, narcissus latent virus (NLV), in leaf extracts from bulbous irises. In addition to the potyvirus coat protein, specific bands identical to those obtained from purified cylindrical inclusions were revealed. For NLV, a specific band was detected in addition to that of the virus coat protein. The method was employed to test about 100 plants and yielded reproducible results.