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Disease Detection and Losses

Enzyme-Linked Immunosorbent Assay of Viruses Infecting Forage Legumes. M. R. McLaughlin, Former visiting assistant professor, Department of Plant Pathology and Physiology, Clemson University, Clemson, SC 29631, Present address of senior author: Research plant pathologist, U. S. Department of Agriculture, Agricultural Research Service, Crop Science Research Laboratory, Department of Plant Pathology and Weed Science, Mississippi State University, P. O. Drawer PG, Mississippi State 39762; O. W. Barnett(2), P. B. Gibson(3), and P. M. Burrows(4). (2)Professor, Department of Plant Pathology and Physiology, Clemson University, Clemson, SC 29631; (3)Research agronomist, U. S. Department of Agriculture, Agricultural Research Service, Department of Agronomy, Clemson University, Clemson, SC 29631; (4)Professor, Experimental Statistics Unit, Clemson University, Clemson, SC 29631. Phytopathology 74:965-969. Accepted for publication 23 March 1984. This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 1984. DOI: 10.1094/Phyto-74-965.

Enzyme-linked immunosorbent assay (ELISA) was adapted for detection of alfalfa mosaic, bean yellow mosaic (BYMV), clover yellow mosaic, clover yellow vein (CYVV), peanut stunt, red clover vein mosaic and white clover mosaic viruses. ELISA was versatile, practical and reliable in indexing forage legumes for viruses, and also differentiated between BYMV and CYVV. ELISA was well suited for large-scale screening programs, and its potential was further extended by mailing sensitized ELISA plates between cooperating laboratories.