VIEW ARTICLE

Physiology and Biochemistry

Reaction of Antiserum Against SDS-Dissociated Rice Dwarf Virus and a Polypeptide of Rice Gall Dwarf Virus. Makoto Matsuoka, Department of Molecular Biology, National Institute of Agrobiological Resources; Yuzo Minobe(2), and Toshihiro Omura(3). (2)Department of Molecular Biology, National Institute of Agrobiological Resources; (3)Department of Plant Protection, National Agriculture Research Center, Tsukuba Science City, Ibaraki 305, Japan. Phytopathology 75:1125-1127. Accepted for publication 10 May 1985. Copyright 1985 The American Phytopathological Society. DOI: 10.1094/Phyto-75-1125.

A serological relationship between rice dwarf (RDV) and rice gall dwarf (RGDV) viruses was found with antiserum against virus dissociated with sodium dodecylsulfate (SDS) by the immune blotting technique. The antiserum against SDS-dissociated RDV, which recognized all peptide components of RDV, cross-reacted with one peptide component of RGDV on immune blotting, while antiserum to intact RDV did not react with RGDV. This immune blotting technique with antibody against SDS-dissociated virus could be a highly sensitive method for studying distant serological relationships among viruses.