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In Vitro Inoculation of Western White Pine Tissue Culture Propagules with Vegetative Hyphae of Cronartium ribicola. Alex M. Diner, BioSource Institute and Department of Forestry, Michigan Technological University, Houghton 49931; Ralph L. Mott, Department of Botany, North Carolina State University, Raleigh 27695. Phytopathology 75:1130-1131. Accepted for publication 16 May 1985. Copyright 1985 The American Phytopathological Society. DOI: 10.1094/Phyto-75-1130.

Propagules of western white pine were inoculated in vitro through intact needles and shoot apex wounds with vegetative hyphae of the blister rust fungus grown from basidiospores in axenic culture. Successfully inoculated propagules showed intracellular haustoria, characteristic of natural inoculation by basidiospores. The opportunity to challenge different tissues of selected host genotypes with cloned replicates of specific rust isolates in vitro may be useful in studying basic processes of resistance and pathogenesis.