VIEW ARTICLE

Physiology and Biochemistry

Differentiation of Postharvest Soft Rotting Bacteria with Two-Dimensional Polyacrylamide Gel Electrophoresis. H. E. Moline, USDA, ARS, Horticultural Science Institute, Horticultural Crops Quality Laboratory, BARC-West, Beltsville, MD 20705; Phytopathology 75:549-553. Accepted for publication 25 November 1984. This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 1985. DOI: 10.1094/Phyto-75-549.

Two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) of acidic ribosome-enriched proteins was used to differentiate several strains of Erwinia and Pseudomonas. Thirty-five erwinias including E. carotovora pv. carotovora, E. carotovora pv. atroseptica, E. chrysanthemi, E. rhapontici, E. amylovora, E. herbicola, and unknown soft rotting strains were compared to strains of Pseudomonas fluorescens. Soft rotting bacterial strains could be readily differentiated with this technique; E.c. carotovora and E.c. atroseptica were consistently distinct enough to be separated into two species and unknown soft rotting isolates could be identified.