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Etiology

Clover Yellow Vein Virus in Limonium sinuatum. R. H. Lawson, Plant pathologist, U. S. Department of Agriculture, Agricultural Research Service, Beltsville, MD 20705; M. D. Brannigan(2), and J. Foster(3). (2)(3)Plant pathologists, U. S. Department of Agriculture, Agricultural Research Service, Beltsville, MD 20705. Phytopathology 75:899-906. Accepted for publication 7 March 1985. This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 1985. DOI: 10.1094/Phyto-75-899.

A potyvirus from statice, Limonium sinuatum, imported from Colombia, was identified as a strain of clover yellow vein virus (CYVV-St) by serology and immunosorbent electron microscopy (IEM). Two isolates of CYVV-St induced reactions of different severity in several hosts, but the two isolates were serologically indistinguishable. The host ranges of both isolates were more similar to the white clover isolate of CYVV from Pratt (CYVV-P) than to bean yellow mosaic virus (BYMV) from gladiolus (BYMV-G, BYMV-G82-25) and red clover (BYMV-204-1). CYVV-St was partially purified from Chenopodium quinoa with chloroform, carbon tetrachloride extraction, and differential centrifugation. Concentration of the virus with PEG 8000 resulted in aggregation and reduced yields. The virus was further purified by equilibrium centrifugation in Cs₂SO₄ but was unstable in CsCl. The normal length of purified virions was 777 nm. Infected leaves of C. quinoa contained cytoplasmic and nuclear inclusions. In agar gel double-diffusion, indirect ELISA, double antibody sandwich ELISA, and immunosorbent electron microscopy tests CYVV-St showed a closer serological relationship to CYVV-P than to BYMV:204-1 and a more distant relationship to BYMV-G and BYMV-G82-25. Antiserum to statice virus Y (Sta VY) from Germany reacted more strongly with CYVV-P and CYVV-St than with the BYMV isolates. We conclude that CYVV-St and Sta VY are strains of CYVV related to BYMV-CYVV-pea mosaic viruses and that CYVV-St is more closely related to BYMV-204-1 than to the gladiolus isolates of BYMV.