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Production and Use of Antibodies from Hen Eggs for the SGV Isolate of Barley Yellow Dwarf Virus. J. S. Hu, Graduate research assistant, Department of Plant Pathology, Cornell University, Ithaca, NY 14853; W. F. Rochow(2), and R. R. Dietert(3). (2)Research plant pathologist, Agricultural Research Service, U.S. Department of Agriculture, and professor, Department of Plant Pathology, Cornell University, Ithaca, NY 14853; (3)Associate professor, Department of Poultry and Avian Sciences, Cornell University, Ithaca, NY 14853. Phytopathology 75:914-919. Accepted for publication 12 March 1985. This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 1985. DOI: 10.1094/Phyto-75-914.

Antibodies were separated from yolks of 216 eggs produced by a 'Babcock 300' hen immunized with less than 100 μg of the SGV isolate of barley yellow dwarf virus (BYDV). Although antibody titer was relatively low, it remained reasonably constant during the 15 mo of egg collection. Only purified immunoglobulin was useful in the coating step of direct (double antibody sandwich) enzyme immunosorbent assays (EIA). Unpurified anti-SGV immunoglobulin was useful in indirect EIA based on trapping SGV with a monoclonal antibody that reacts with both SGV and the MAV isolate. Five kinds of serological tests showed that SGV is related to MAV and PAV, but distinct from RPV and RMV, four other isolates of BYDV used for comparison. The indirect EIA provided an assay for SGV in the presence of PAV, so we were able to determine that PAV is not a helper virus for dependent transmission of SGV by the aphid Rhopalosiphum padi from mixed infections of SGV and PAV.