VIEW ARTICLE

Etiology

Some Biological and Physicochemical Properties of Bean Rugose Mosaic Virus. O. Acosta, Department of Physiological Sciences, Facultad de Medicina, Universidad Nacional, Bogotá, Colombia; A. Alegría(2), and R. Lastra(3). (2)Department of Biology, Facultad de Ciencias, Universidad del Valle, Cali, Colombia; (3)Laboratory of Plant Viruses, Instituto Venezolano de Investigaciones Cientificas, Caracas, Venezuela. Phytopathology 76:1182-1189. Accepted for publication 4 December 1985. Copyright 1986 The American Phytopathological Society. DOI: 10.1094/Phyto-76-1182.

Characterization of a bean rugose mosaic virus (BRMV) isolate from Cali, Colombia, confirmed that this virus belongs to the comovirus group. In sucrose density gradients BRMV separated into three centrifugal components after rate zonal sedimentation and into four or five density components after CsCl equilibrium sedimentation, depending on the type of buffer. The native nucleoprotein particles also resolved into one or two electrophoretic forms depending on the type of buffer used for purification. Unfractionated BRMV, as well as the individual nucleoprotein particles, contained two (39.5 x 10³ and 24.5 x 10³ daltons) or three (39.5 x 10³, 24.5 x 10³ and 22 x 10³ daltons) proteins that could be separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The number of proteins depended on the type of buffer used for the virion purification. From symptomatology and biophysical characteristics the Colombian isolate seemed to be closely related to the BRMV-Costa Rica strain but somewhat different from the BRMV-Ampollado strain from El Salvador.