VIEW ARTICLE

Techniques

Construction of an Inexpensive ELISA Plate Reader. L. W. Stobbs, Research scientist, Agriculture Canada, Research Station, Vineland Station, Ontario L0R 2E0; Phytopathology 76:1217-1221. Accepted for publication 26 May 1986. Copyright 1986 Department of Agriculture, Government of Canada. DOI: 10.1094/Phyto-76-1217.

An inexpensive enzyme-linked immunosorbent assay (ELISA) plate reader was constructed by interfacing a solid-state photometer with a light microscope. By using the light path system of the microscope, only a light-sensing circuit and narrow bandpass filter were necessary to obtain reliable ELISA substrate readings. Laboratory tests have shown that this unit provides accuracy, reproducibility, and linearity comparable to those of a state-of-the-art commercial unit.