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Molecular Plant Pathology

Transformation of Escherichia coli by Plasmid DNA from Erwinia stewartii. R. D. Frederick, Department of Plant Pathology, The Ohio State University, Ohio Agricultural Research and Development Center, Wooster 44691, Present address: Department of Plant Pathology, The University of California, Berkeley 94720; D. L. Coplin, Department of Plant Pathology, The Ohio State University, Ohio Agricultural Research and Development Center, Wooster 44691. Phytopathology 76:1353-1356. Accepted for publication 30 June 1986. Copyright 1986 The American Phytopathological Society. DOI: 10.1094/Phyto-76-1353.

Seven cryptic plasmids from Erwinia stewartii strain DC336 were transformed into Escherichia coli strain HB101 using indirect selection for R-plasmid pSC201. Restriction endonuclease digestions of these plasmids revealed that they are unique and the larger plasmids are not cointegrates or multimers of the smaller plasmids. Hybridization experiments showed that pDC150 and pDC170 sequences are conserved in similar-sized plasmids in other strains. pDC150 and pDC170 also contained a sequence(s) that hybridized weakly to many other E. stewartii plasmids.