VIEW ARTICLE

Techniques

Histochemical Localization of Juglone and Related Constituents of Pecan. Clinton H. Graves, Professor of plant pathology, Mississippi State University, Mississippi State 39762; Matthew W. MacGown(2), Paul A. Hedin(3), and T. H. Filer(4). (2)Research associate, Mississippi State University, Mississippi State 39762; (3)Research chemist, Boll Weevil Research Laboratory, USDA-ARS, Mississippi State, MS 39762; and (4)Leader, Southern Forest Experiment Station, Stoneville, MS 38776. Phytopathology 76:205-208. Accepted for publication 7 September 1985. Copyright 1986 The American Phytopathological Society. DOI: 10.1094/Phyto-76-205.

Potential methods for selectively demonstrating juglone and related compounds in pecan (Carya illinoensis) tissue were studied and the most effective method was outlined. Fixation with formaldehyde vapor followed by the Hoepfner-Vorsatz test for phenolics, as modified by Reeve, provided the most reliable color reaction.