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Etiology

Structural Comparison of Poa Semilatent Virus and Barley Stripe Mosaic Virus. B. G. Hunter, Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907; L. A. Heaton(2), C. E. Bracker(3), and A. O. Jackson(4). (2)(3)(4)Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907. Phytopathology 76:322-326. Accepted for publication 23 October 1985. Copyright 1986 The American Phytopathological Society. DOI: 10.1094/Phyto-76-322.

Barley stripe mosaic virus (BSMV) and poa semilatent virus (PSLV) are members of the hordeivirus group. The physicochemical data presented here confirm the morphological similarity of PSLV and BSMV. The two viruses can also be distinguished serologically, and their capsid proteins differ in mobility in polyacrylamide gels, PSLV coat protein has a relative molecular mass (M_r) of 23,000, but BSMV coat protein has an M_r of 25,000. Both viruses contain multiple RNA species, but after denaturation the three RNAs of PSLV migrate more slowly in agarose gels than the three RNAs of the ND18 strain of BSMV. The genomes of the two viruses have no significant nucleotide sequence homology as measured by nucleic acid hybridization. Thus, these results support previous suggestions that BSMV and PSLV are distinct members of the same group.