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Etiology

Serological Relationships and In Vitro Translation of an Antigenically Distinct Strain of Papaya Ringspot Virus. L. Quiot- Douine, Former visiting scientist, Department of Plant Pathology, University of Florida, Gainesville 32611, Present address: I.N.R.A. Virologie, G.E.R.D.A.T. BP 5035, Avenue du Val de Montferrand, 34032 Montpellier, France; D. E. Purcifull(2), E. Hiebert(3), and M. V. G. de Mejia(4). (2)(3)Professor, professor, respectively, Department of Plant Pathology, University of Florida, Gainesville 32611; (4)Former graduate student, Department of Plant Pathology, University of Florida, Gainesville 32611, Present address: Departmento de Biologia, Escuela de Ciencias, Universidad de Oriente, Cumana, Estado Sucre, Venezuela 6101. Phytopathology 76:346-351. Accepted for publication 25 September 1985. Copyright 1986 The American Phytopathological Society. DOI: 10.1094/Phyto-76-346.

The serological relationships between a potyvirus isolated from squash in Guadeloupe (isolate Q10), a watermelon mosaic virus-1 isolate of papaya ringspot virus (PRSV-W), a papaya isolate of papaya ringspot virus, and a virus isolated from cucurbits in Morocco (WMV-M) were analyzed by sodium dodecyl sulfate immunodiffusion tests with inclusion body protein and coat protein antisera. The products of in vitro translation of purified Q10-RNA were also analyzed by immunoprecipitation. A 114k product obtained in the rabbit reticulocyte lysate (RRL) system and a 51k product obtained in both the RRL and the wheat germ systems were immunoprecipitated by PRSV-W amorphous inclusion protein antiserum. A 70k product and a 36k product obtained in the lysate system were precipitated by PRSV-W cylindrical inclusion protein antiserum and by isolate Q10 coat protein antiserum, respectively. The SDS-immunodiffusion tests showed that the amorphous inclusions of isolate Q10 and PRSV-W were serologically closely related, that their cylindrical inclusions had at least one common antigenic determinant and one specific determinant, and that isolate Q10 coat protein had at least three antigenic determinants: one specific to isolate Q10; one that was common to isolate Q10 and PRSV-W; and a third one that was common to isolate Q10, PRSV-W, and WMV-M. Since most of its properties were similar to those of PRSV-W, isolate Q10 is considered to be a serologically distinct strain of papaya ringspot virus.