VIEW ARTICLE

Techniques

Vesicular-Arbuscular Mycorrhizal Infection in Transformed Root-Inducing T-DNA Roots Grown Axenically. J. Mugnier, Rhône-Poulenc Agrochimie, 69263 Lyon Cédex 9, France; B. Mosse, Rhône-Poulenc Agrochimie, 69263 Lyon Cédex 9, France, Present address: La Fouillarge, 24320 Lusignac/Verteillac, France. Phytopathology 77:1045-1050. Accepted for publication 13 January, 1987. Copyright 1987 The American Phytopathological Society. DOI: 10.1094/Phyto-77-1045.

Details are given of a technique for obtaining typical vesicular-arbuscular mycorrhizal infections in root organ cultures of Convolvulus sepium transformed by root-inducing T-DNA from Agrobacterium rhizogenes. A bicompartmental culture system was used. Roots, growing from a modified Murashige and Skoog nutrient medium and sugar into a compartment containing water agar and neutralized peat, were infected with germinated spores of Glomus mosseae. The uses of such a system for studies of nutrient exchange between plant and fungus are discussed.