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Etiology

Concentration of Maize Chlorotic Mottle Virus Increased in Mixed Infections with Maize Dwarf Mosaic Virus, Strain B. Karen -Beth Goldberg, Agricultural Research Service, U.S. Department of Agriculture and Department of Plant Pathology, Nebraska Agricultural Experiment Station, University of Nebraska, Lincoln, 68583, Present address: Plant Pathology Department, University of Kentucky, Lexington, 40546; Myron K. Brakke, Agricultural Research Service, U.S. Department of Agriculture and Department of Plant Pathology, Nebraska Agricultural Experiment Station, University of Nebraska, Lincoln, 68583. Phytopathology 77:162-167. Accepted for publication 18 June 1986. This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 1987.. DOI: 10.1094/Phyto-77-162.

The concentration of maize chlorotic mottle virus (MCMV) was up to 5.4 times higher in plants infected with both MCMV and maize dwarf mosaic virus, strain B (MDMV-B), than in plants infected with MCMV only. The concentration of MDMV-B was the same in doubly and singly infected plants. Plants infected with both viruses had a reduced level of chlorophyll and a lower than normal ratio of chloroplast to cytoplasmic rRNA. Purified MCMV had an extinction coefficient of 6.7 cm² mg^–1 at 260 nm, an absorption maximum at 258 nm, minimum at 240 nm, and 25% RNA.