VIEW ARTICLE

Etiology

Detection and Differentiation of Maize Dwarf Mosaic Virus, Strains A and B, by Use of Different Class Immunoglobulins in a Double-Antibody Sandwich Enyzme-Linked Immunosorbent Assay. F. E. Jones, Department of Plant Pathology, Iowa State University, Ames 50011; J. H. Hill(2), and D. P. Durand(3). (2)Departments of Plant Pathology and Microbiology, Iowa State University, Ames; (3)Department of Microbiology, Iowa State University, Ames. Phytopathology 78:1118-1124. Accepted for publication 3 March 1988. Copyright 1988 The American Phytopathological Society. DOI: 10.1094/Phyto-78-1118.

Murine monoclonal antibodies of two classes and reactive with apparently different epitopes on particles of the A and B strains of maize dwarf mosaic virus (MDMV) were used in a capture indirect enzyme-linked immunosorbent assay (ELISA). The immunoassay used IgM capture and IgG second antibodies. This allowed use of an IgG-specific alkaline phosphatase-conjugated antibody to detect MDMV-A- and MDMV-B-bound IgG. Discriminatory capacity of the assay, tested in sap from leaves that were infected with one of several different strains of MDMV and sugarcane mosaic virus (SCMV), was generally sufficient to identify the A and B strains of MDMV, although some cross-reactivity was observed among certain virus strains.