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Anti-Idiotypic Antibodies Against an Anti-Barley Yellow Dwarf Virus Monoclonal Antibody. J. S. Hu, Department of Plant Pathology, Cornell University, Ithaca, NY 14853, Present address: Department of Plant Pathology, New York State Agricultural Experiment Station, Cornell University, Geneva 14456; W. F. Rochow, Department of Plant Pathology, Cornell University, Ithaca, NY 14853. Phytopathology 78:1302-1307. Accepted for publication 28 April 1988. This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 1988. DOI: 10.1094/Phyto-78-1302.

Antisera were produced in rabbits against purified anti-RPV monoclonal antibody (mAB-RPV1), which neutralizes RPV transmission by Rhopalosiphum padi. Active anti-idiotypic antibodies were purified from the rabbit antisera with a protein A-Sepharose column. After immunoprecipitation with both mouse serum and mouse IgG, the antibodies reacted with mAB-RPV1 but not with several other anti-RPV monoclonal antibodies of the same subclass. The antibodies inhibited reactions between RPV and mAB-RPV1; the reactions between mAB-RPV1 and the antibodies also were inhibited by RPV in competitive enzyme immunosorbent assays. When the antibodies were injected into a rabbit, active anti-RPV (= anti-anti-idiotypic) antiserum was produced. We conclude that true anti-idiotypic antibodies against mAB-RPV1 were produced.