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Molecular Plant Pathology

Analysis of Sowthistle Yellow Vein Virus-Specific RNAs in Infected Hosts. D. C. Stenger, Graduate research assistant, Department of Plant Pathology, University of California, Berkeley 94720; J. Richardson(2), E. S. Sylvester(3), A. O. Jackson(4), and T. J. Morris(5). (2)(3)Staff research associate, and professor, Division of Entomological Sciences, University of California, Berkeley 94720; (4)(5)Department of Plant Pathology, University of California, Berkeley 94720. Phytopathology 78:1473-1477. Accepted for publication 1 July 1988. Copyright 1988 The American Phytopathological Society. DOI: 10.1094/Phyto-78-1473.

Recombinant plasmids containing sequences derived from the genome of sowthistle yellow vein virus (SYVV) were constructed and used as probes in northern blots to analyze viral-specific RNAs extracted from infected plants and aphids. Recombinant plasmid probes hybridized to a 13-kb, genome-size RNA present in extracts from infected but not uninfected hosts. Four distinct size classes of polyadenylated RNAs were also detected in infected plant extracts. No sequence relatedness was detected between the genomic RNAs of SYVV and another plant rhabdovirus. Sonchus yellow net virus (SYNV), in northern blots by using plasmids containing SYVV or SYNV sequences, or DNA complimentary to SYNV RNA as probes. SYVV plasmid probes detected SYVV infection of individual aphids in dot-hybridization assays.