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Techniques

Detection of Coronatine Toxin of Pseudomonas syringae pv. glycinea with an Enzyme-Linked Immunosorbent Assay. J. V. Leary, Department of Plant Pathology, University of California, Riverside 92521; S. Roberts(2), and J. W. Willis(3). (2)(3)Department of Plant Pathology, University of California, Riverside 92521, (3)Present address: EniChem, Monmouth Junction, NJ 08852. Phytopathology 78:1498-1500. Accepted for publication 19 July 1988. Copyright 1988 The American Phytopathological Society. DOI: 10.1094/Phyto-78-1498.

A rapid, reliable method for detecting coronatine toxin produced by Pseudomonas syringae pv. glycinea employed antibodies raised to a bovine serum albumin-coronatine conjugate. The antiserum was used in an enzyme-linked immunosorbent assay to detect coronatine in culture filtrates and partially purified toxin preparations.