VIEW ARTICLE

Cytology and Histology

Barley Stripe Mosaic Virus but Not Brome Mosaic Virus Binds to Wheat Streak Mosaic Virus Cylindrical Inclusions In Vivo. W. G. Langenberg, Agricultural Research Service, U.S. Department of Agriculture, and Department of Plant Pathology, University of Nebraska, Lincoln 68583; Phytopathology 78:589-594. Accepted for publication 4 November 1987. This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 1988. DOI: 10.1094/Phyto-78-589.

Immunogold detection was used to follow interactions of three wheat viruses in doubly infected tissue. Most cylindrical inclusions (CI) of wheat streak mosaic virus (WSMV) in young, doubly infected cells were labeled with antibody to barley stripe mosaic virus (BSMV) or antibody to WSMV but not with antibody to brome mosaic virus (BMV), indicating an association of BSMV or WSMV with WSMV CI. Antibody to BSMV-coat protein absorbed with an excess of intact BSMV virions no longer labeled WSMV CI but still labeled BSMV virus aggregates, indicating that intact virions rather than coat protein adsorbed to CI. The amount of BSMV and WSMV virions and cylindrical inclusions was about the same in doubly infected as in singly infected plants whether analyzed by electron microscopy or by gel electrophoresis of extracts from infected tissue. Interactions between the two viruses were not detectably influenced by greenhouse temperatures or light intensity. Cylindrical inclusions of WSMV were not labeled with monoclonal antibodies to six contractile proteins that are widely conserved in the plant and animal kingdoms.