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VIEW ARTICLE
Etiology
Characterization of the Oat-Infecting Strain of Maize Dwarf Mosaic Virus. L. L. McDaniel, Former postdoctoral associate, Department of Plant Pathology, Ohio Agricultural Research and Development Center (OARDC), The Ohio State University (OSU), Wooster 44691, Present address: American Type Culture Collection, 12301 Parklawn Dr., Rockville, MD 20852; D. T. Gordon, professor, Department of Plant Pathology, Ohio Agricultural Research and Development Center (OARDC), The Ohio State University (OSU), Wooster 44691. Phytopathology 79:113-120. Accepted for publication 22 August 1988. Copyright 1989 The American Phytopathological Society. DOI: 10.1094/Phyto-79-113.
Virions of an oat-infecting strain of maize dwarf mosaic virus (MDMV-O) were purified using 0.01 M Tris-citrate, pH 7.0, by differential centrifugation, rate-zonal centrifugation in sucrose gradients, and isopycnic banding in CsCl gradients. Satisfactory virion resuspension after high-speed centrifugation also was obtained with 0.01 M potassium phosphate, pH 7.4, containing 1-3 M urea. Purified virions measured 650 to 808 x 14 nm and had an s20,w of 168 S and a buoyant density of 1.297 g/ml in CsCl. Viral nucleic acid was digested by RNase but not DNase, and virions contained 6.6% RNA by weight as determined by ultraviolet absorbance. Three virion capsid-protein subunits were detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The molecular masses (Mr) of the three protein subunits were estimated to be 35.1, 33.0, and 30.5 kDa. In contrast, single capsid-protein subunits were observed for virions of MDMV strains A, D, E, and F in SDS-PAGE, whereas three were observed for MDMV-B virions with Mr similar to those for the virion capsid-protein subunits of MDMV-O. Serologically, MDMV-O was distantly related to strains A, B, D, and F of MDMV as determined by enzyme-linked immunosorbent, immunodotblot, and microprecipitin assays, and to MDMV strain E and sugarcane mosaic virus strain A, but not H, as determined by the microprecipitin assay.
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